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| Status |
Public on Apr 26, 2018 |
| Title |
Self-recognition of an inducible host lncRNA by RIG-I feedback restricts innate immune response |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we employed a combination of UV-RIP-seq, PacBio-seq and iCLIP-seq technologies to identify long non-coding RNA associated with cytoplasmic RIG-I protein in mouse macrophages cells.
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| Overall design |
Two UV-RIP were performed using anti-Flag antibody in RAW264.7 cells stably expressing Flag-tagged RIG-I with or without vesicular stomatitis virus (VSV) infection for 12 hr, then co-purified RNAs were analyzed using Illumina HiSeq4000 sequencing. One RIP was performed using anti-RIG-I antibody in RAW264.7 cells with VSV infection for 12 hr, then co-purified RNAs were sequenced using PacBio’s SMRT long-read sequencing strategy. Total RNA from mouse peritoneal macrophages was analyzed using PacBio sequencing. iCLIP was performed using anti-RIG-I antibody in RAW264.7 cells upon VSV infection for 12 hr and then made Illumina HiSeq2500 SE50 Sequencing.
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| Contributor(s) |
Jiang M, Zhang S, Yang Z, Lin H, Zhu J, Liu L, Wang W, Liu S, Liu W, Ma Y, Zhang L, Cao X |
| Citation(s) |
29706547 |
| |
| Submission date |
Oct 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Minghong Jiang |
| E-mail(s) |
jiangminghong@163.com
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| Organization name |
Chinese Academy of Medical Sciences
|
| Street address |
5 Dong Dan San Tiao
|
| City |
Beijing |
| ZIP/Postal code |
100005 |
| Country |
China |
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| Platforms (3) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA416003 |
| SRA |
SRP121994 |
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