|
| Status |
Public on May 31, 2018 |
| Title |
Influenza virus facilitates its infectivity by activating p53 to inhibit the expression of interferon-induced transmembrane proteins (IFITMs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptome analysis of mock or H1N1 IAV PR8 infected p53WT A549 and p53null A549-KO3 cells by Affymetrix GeneChip Human Transcriptome 2.0 Arrays to achieve a set of genes those are regulated by p53 and responsive to IAV infection.
Influenza A virus infection activates cellular p53, however it has not been clear whether this process has pro- or anti- viral effects. In this study, using human isogenic p53 wildtype A549 cells and p53null A549-KO3 cells generated from the CRISPR/Cas9 technology, we report that p53null cells exhibit significantly reduced viral propagation property when infected with influenza A virus (H1N1/A/Puerto Rico/8/34). Here, using genome-wide microarray analysis we revealed that p53 regulates the expression of a large set of interferon-inducible genes, some of which are directly associated with viral infectivity and later experimentally validated to be responsible for p53-regulated IAV infectivity.
|
| |
|
| Overall design |
We analyzed the gene expression profiling of H1N1 infected p53WT A549 and p53null A549-KO3 cells generated by CRISPR/Cas9 technology using Affymetrix Human Transciptome 2.0 Array with three technical replicates in each condition.
|
| Web link |
https://www.frontiersin.org/articles/10.3389/fimmu.2018.01193/full
|
| |
|
| Contributor(s) |
Wang B, Ye Z, Lam T, Chen J, Soh M, Ren E |
| Citation(s) |
29904383 |
| Submission date |
Oct 27, 2017 |
| Last update date |
Oct 29, 2018 |
| Contact name |
jinmiao chen |
| E-mail(s) |
chen_jinmiao@immunol.a-star.edu.sg
|
| Organization name |
Singapore Immunology Network
|
| Street address |
8A Biomedical Grove, #04-06 Immunos
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA416117 |
Less...
More...