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Series GSE106385 Query DataSets for GSE106385
Status Public on Feb 12, 2019
Title Gene expression profiles in experimental uremic cardiomyopathy.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To define differentially regulated pathways in heart tissue from mice with chornic kidney disease (CKD) compared to age-matched controls.
Methods: CKD was induced in 5-week-old, male 129X1/SvJ mice (JAX) through five-sixths nephrectomy in a two-step surgery (n=5). Age-matched mice undergoing bilateral sham surgeries served as controls (n=5). Heart tissue was collected at 8 weeks of CKD for next generation sequencing.
Results: Hearts from mice with uremic cardiomyopathy yielded over 1,000 differentially-expressed mRNA transcripts compared to hearts from age-matched, sham-operated mice. Ingenuity biofunctions analysis identified significant enrichment for genes involved in Tissue Morphology, Immune Cell Trafficking, Cardiovascular Development and Function, and Humoral Immune Response. We focused on Ingenuity canonical pathways involving inflammation and immune system function including pathways needed for a T-cell mediated response: leukocyte extravasation, antigen presentation, dendritic cell maturation, T cell co-stimulatory signaling, and T-helper cell differentiation.
Conclusions: Left ventricles from mice with CKD display differential expression in a number of pathways suggesting inflammation and surprisingly involved genes involved in the adaptive immune system.
Overall design Left ventricular mRNA profiles from 5 mice with CKD and 5 normal mice were generated using next generation RNA sequencing on the Illumina HiSeq 1000.
Contributor(s) Winterberg PD, Patel N, Tharp GK
Citation(s) 30728178
Submission date Oct 31, 2017
Last update date May 15, 2019
Contact name Gregory K Tharp
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
Platforms (1)
GPL15103 Illumina HiSeq 1000 (Mus musculus)
Samples (10)
GSM2836985 RKM-012-01
GSM2836986 RKM-012-02
GSM2836987 RKM-012-03
BioProject PRJNA416660
SRA SRP123230

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Supplementary file Size Download File type/resource
GSE106385_165_Winterberg_FPKM_exprs.txt.gz 726.2 Kb (ftp)(http) TXT
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Processed data are available on Series record

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