|
| Status |
Public on Oct 30, 2018 |
| Title |
RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes after UCA1 knockdown in human erythroid cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goals of this study are to determine possible downstream targets of UCA1 on day8 of erythroid differentiation with or without UCA1 knockdown.
Methods:gene expression profiling with or without UCA1 knockdown in differentiated erythroblasts at day8 were generated by deep sequencing, induplicate, using Illumina GAIIx.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays.
Conclusions: heme metabolism genes were down-regulated after UCA1 knockdown during erythroid differentiation
|
| |
|
| Overall design |
mRNA profiles of day8 erythroid cells with or without UCA1 knockdown were generated by deep sequencing, in duplicate, using Illumina GAIIx.
|
| |
|
| Contributor(s) |
Shi L, Liu J |
| Citation(s) |
30349036 |
| |
| Submission date |
Nov 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hua Jin Liu |
| E-mail(s) |
liufeng198308@yeah.net
|
| Phone |
15202208670
|
| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Department |
State Key Laboratory of Experimental Hematology
|
| Street address |
288 Nanjing Road
|
| City |
TianJin |
| ZIP/Postal code |
300020 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE106567 |
Long Non-Coding RNA-Dependent Mechanism to Control Heme Biosynthesis and Erythrocyte Maturation |
|
| Relations |
| BioProject |
PRJNA417183 |
| SRA |
SRP124072 |
Less...
More...