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| Status |
Public on Mar 08, 2018 |
| Title |
Genome-wide sequencing and comparative profiling of cattle sperm DNA methylome |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Using whole-genome bisulfite sequencing (WGBS), we profiled the DNA methylome of cattle sperms through comparison with three bovine somatic tissues (mammary grand, brain and blood). Large differences between cattle sperms and somatic tissues were observed in the methylation patterns.
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| Overall design |
Genomic DNA for each tissue was isolated according to the QIAamp DNA Mini Kit protocol (QIAGEN, Valencia, CA, USA). The quality of DNA samples was evaluated using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) including degradation, and potential RNA contamination, and purity (OD260/OD280), and concentration using a spectrophotometer (NanoDrop Technologies, Rockland, DE) to meet the requirements for library construction. The qualified genomic DNA from somatic tissues and sperm were used to construct a library. The library different lanes of a HiSeq X Ten (Illumina, San Diego, CA, USA) to generate 150-bp paired-end reads by Novogene (Novogene, Beijing, China).
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| Contributor(s) |
Zhou Y, Liu GE |
| Citation(s) |
30810461, 29635292, 34968233 |
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| Submission date |
Nov 05, 2017 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Yang Zhou |
| E-mail(s) |
yangzhou@mail.hzau.edu.cn
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| Phone |
18162530091
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| Organization name |
Huazhong Agricultural University
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| Department |
College of animal science
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| Street address |
Shizishanjie #1
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA417285 |
| SRA |
SRP124288 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106538_RAW.tar |
2.9 Gb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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