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| Status |
Public on Jun 04, 2018 |
| Title |
DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Lung CD4+ T cell responses may determine outcomes in pediatric pnuemonia. We therefore sought to define the transcriptional response and underlying DNA methyaltion landscape of lung CD4+ T cells by treating neonatal (~5 days of age) and juvenile (~15 days of age) mice with 2.8 million CFUs of E. coli or PBS (control) by aspiration. 48 hours later, lung CD4+ T cells were isolated by magnetic bead pre-enrichment and fluorescence-activated cell sorting for RNA-sequencing (RNA-seq) and modified reduced representation bisulfite sequencing (mRRBS).
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| Overall design |
3 replicates per group, each representing pooled cells from 3 neonatal and 2 juvenile mice/replicate.
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| |
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| Contributor(s) |
Singer BD, McGrath-Marrow SA |
| Citation(s) |
29866884 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K08 HL128867 |
Regulatory T cell Uhrf1 and DNA methylation in repair of acute lung injury |
NORTHWESTERN UNIVERSITY AT CHICAGO |
Benjamin David Singer |
| R01 HL114800 |
Resolution of Diffuse Inflammatory Lung Injury in Neonatal Mice |
JOHNS HOPKINS UNIVERSITY |
Sharon Ann McGrath-Morrow |
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| |
| Submission date |
Nov 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Benjamin D. Singer |
| E-mail(s) |
benjamin-singer@northwestern.edu
|
| Organization name |
Northwestern University Feinberg School of Medicine
|
| Street address |
303 E. Superior St., Simpson Querrey 5th floor
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE106807 |
DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice |
|
| Relations |
| BioProject |
PRJNA418112 |
| SRA |
SRP124836 |
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