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Series GSE106806 Query DataSets for GSE106806
Status Public on Jun 04, 2018
Title DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Lung CD4+ T cell responses may determine outcomes in pediatric pnuemonia. We therefore sought to define the transcriptional response and underlying DNA methyaltion landscape of lung CD4+ T cells by treating neonatal (~5 days of age) and juvenile (~15 days of age) mice with 2.8 million CFUs of E. coli or PBS (control) by aspiration. 48 hours later, lung CD4+ T cells were isolated by magnetic bead pre-enrichment and fluorescence-activated cell sorting for RNA-sequencing (RNA-seq) and modified reduced representation bisulfite sequencing (mRRBS).
Overall design 3 replicates per group, each representing pooled cells from 3 neonatal and 2 juvenile mice/replicate.
Contributor(s) Singer BD, McGrath-Marrow SA
Citation(s) 29866884
NIH grant(s)
Grant ID Grant title Affiliation Name
K08 HL128867 Regulatory T cell Uhrf1 and DNA methylation in repair of acute lung injury NORTHWESTERN UNIVERSITY AT CHICAGO Benjamin David Singer
R01 HL114800 Resolution of Diffuse Inflammatory Lung Injury in Neonatal Mice JOHNS HOPKINS UNIVERSITY Sharon Ann McGrath-Morrow
Submission date Nov 13, 2017
Last update date May 15, 2019
Contact name Benjamin D. Singer
Organization name Northwestern University Feinberg School of Medicine
Street address 303 E. Superior St., Simpson Querrey 5th floor
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM2850573 RRBS_JE1
GSM2850574 RRBS_JE2
GSM2850575 RRBS_JE3
This SubSeries is part of SuperSeries:
GSE106807 DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice
BioProject PRJNA418112
SRA SRP124836

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Supplementary file Size Download File type/resource
GSE106806_RAW.tar 183.8 Mb (http)(custom) TAR (of COV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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