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| Status |
Public on Aug 13, 2018 |
| Title |
Algesic MBP peptide induce pain-specific signaling in rodent Schwann Cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We demonstrated the pain-specific response to the algesic peptide fragment MBP84-104 of myelin basic protein that induces pain if injected into sciatic nerve of rats and mice. We used the wild-type peptide MBP 84-104, H89G mutant peptide (MBP84-104-H89G), scramble peptide (MBP84-104-SCR) and phosphomimetic peptide MBP84-104-mimTT to stimulate the primary rat Schwann cell cultures. After 24h we isolated total RNA and conducted genome wide RNA-seq. In addition, we performed RNA-seq using Schwann cells constitutively expressing an MBP84-104-mCherry construct. The gene expression data was analyzed using Ingenuity Pathway Analysis software. We conclude that the Schwann cells expressing MBP84-104 constructs stimulate pain-specific signaling pathways thus representing a relevant model to study neuropathic pain.
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| Overall design |
examination of gene expression in Schwann cells stimulated by MBP fragment
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| |
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| Contributor(s) |
Chernov AV, Strongin AY, Shubayev V |
| Citation(s) |
30079618 |
| Submission date |
Nov 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrei Chernov |
| E-mail(s) |
achernov@ucsd.edu
|
| Phone |
858 952 2854
|
| Organization name |
University of California San Diego
|
| Department |
Anesthesiology
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| Lab |
Chernov Lab
|
| Street address |
9500 Gilman Dr
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA418791 |
| SRA |
SRP125137 |
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