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| Status |
Public on Feb 26, 2019 |
| Title |
Transcriptome profile of ATP7B KO cells and WT cells treated with Copper (Cu) to study Autophagy as a pro-survival mechanism in Wilson disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand whether hepatic cells use autophagy to defend against Cu toxicity in WD, we employed ATP7B knockout (ATP7B-KO) HepG2 cell line, by Quant-seq experiments. Transcriptome analysis of the of ATP7B KO cells and WT cells treated with Copper (Cu) compared with untreated cells.
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| Overall design |
total RNA was extracted from ATP7B KO cells and WT cells treated with Copper (Cu); RNA extracted from untreated cells was used as control.
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| Contributor(s) |
De Cegli R, Polishchuk R |
| Citation(s) |
30452922 |
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| Submission date |
Nov 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Annamaria Carissimo |
| E-mail(s) |
carissimo@tigem.it
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| Organization name |
Tigem
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| Street address |
via Castellino
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| City |
Napoli |
| ZIP/Postal code |
80131 |
| Country |
Italy |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| GSM2864795 |
Hep G2 wt cells untreated, biological rep1 |
| GSM2864796 |
Hep G2 wt cells untreated, biological rep2 |
| GSM2864797 |
Hep G2 wt cells untreated, biological rep3 |
| GSM2864798 |
Hep G2 wt cells treated with Cu, biological rep1 |
| GSM2864799 |
Hep G2 wt cells treated with Cu, biological rep2 |
| GSM2864800 |
Hep G2 wt cells treated with Cu, biological rep3 |
| GSM2864801 |
ATP7B KO Hep G2 cells untreated, biological rep1 |
| GSM2864802 |
ATP7B KO Hep G2 cells untreated, biological rep2 |
| GSM2864803 |
ATP7B KO Hep G2 cells untreated, biological rep3 |
| GSM2864804 |
ATP7B KO Hep G2 cells treated with Cu, biological rep1 |
| GSM2864805 |
ATP7B KO Hep G2 cells treated with Cu, biological rep2 |
| GSM2864806 |
ATP7B KO Hep G2 cells treated with Cu, biological rep3 |
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| Relations |
| BioProject |
PRJNA419762 |
| SRA |
SRP125604 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE107323_RAW.tar |
2.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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