|
| Status |
Public on Nov 29, 2017 |
| Title |
Small RNA deep sequencing using Illumina HiSeq2500 |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
33 miRNAs significantly decreased and 75 miRNAs significantly increased in BCR-ABL+ LSK compared with BCR-ABL- LSK cells, suggesting distinct BCR-ABL-dependent mechanisms of microRNA regulation.
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| |
|
| Overall design |
LSK cells from non-induced and induced chronic myeloid leukemia (CML) mice were sorted and total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Valencia, CA). Small RNA sequencing was performed using Illumina HiSeq2500 at the COH Integrative Genomics Core following the manufacturer's sample preparation protocol (TruSeq Small RNA Sample Prep kit, Illumina, Inc.) with some modifications.
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| |
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| Contributor(s) |
Zhang B, Zhao D |
| Citation(s) |
29505034 |
| |
| Submission date |
Nov 28, 2017 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Xiwei Wu |
| E-mail(s) |
xwu@coh.org
|
| Organization name |
City of Hope National Medical Center
|
| Department |
Computational and Quantitative Medicine
|
| Street address |
1500 E. Duarte Rd.
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA420069 |
| SRA |
SRP125757 |
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