|
| Status |
Public on Jan 01, 2018 |
| Title |
RNA sequencing of dendritic cells undergoing interaction with T cells in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used RNA sequencing to characterize gene expression of dendritic cells from mouse lymph node that, based on LIPSTIC labeling, underwent interaction with CD4+ T cells.
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| |
|
| Overall design |
Antigen pulsed dendritic cells (DCs) were transferred into recipient mice, followed by antigen specific CD4+ T cells. Forty-eight hours after T cell transfer, endogenous dendritic cells were isolated by facs sorting from mouse lymph node and analyzed based on their in vivo LIPSTIC labeling.
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| |
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| Contributor(s) |
Pasqual G, Schweitzer LD, Cui A, Hacohen N, Victora GD |
| Citation(s) |
29342141 |
| |
| Submission date |
Dec 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Ang Cui |
| E-mail(s) |
angcui@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Street address |
415 Main St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (14)
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|
| Relations |
| BioProject |
PRJNA420958 |
| SRA |
SRP126061 |
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