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Series GSE10800 Query DataSets for GSE10800
Status Public on Mar 13, 2008
Title Genome-wide Binding Site Mapping of Estrogen Receptor alpha and c-MYC in Breast Cancer Cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Estrogens are steroid hormones that play critical roles in the initiation, development, and metastasis of breast and uterine cancers. The estrogen (E2) response in breast cancer cells is predominantly mediated by the estrogen receptor-alpha (ER alpha), a ligand-activated transcription factor. ER alpha regulates transcription of target genes through direct binding to its cognate recognition sites, known as estrogen response elements (EREs), or by modulating the activity of other DNA-bound transcription factors at alternative DNA sequences. The proto-oncogene c-myc is upregulated by ER¦Á in response to E2 and encodes a transcription factor, c-MYC, which regulates a cascade of gene targets whose products mediate cellular transformation. This study aims at mapping the binding sites of these two transcription factors (ER alpha and c-MYC) in one ER alpha positive breast cancer cell line (MCF7 cell line).
Keywords: ChIP-Chip Analysis
 
Overall design This series contains ChIP-on-Chip data sets for two transcription factors (ER alpha and c-MYC) and control samples (INPUT). All the experiments are done in triplicates. MCF7 Cells were E2-deprived for 3 days and then were treated with 10 nM E2 (45 minutes and 2 hours for mapping ER alpha and c-MYC binding sites, respectively) at 80% confluence.
 
Contributor(s) Hua S, White KP
Citation(s) 18414489
Submission date Mar 12, 2008
Last update date Mar 21, 2017
Contact name Sujun Hua
E-mail(s) shua@mdanderson.org
Organization name MD Anderson Cancer Center
Lab Ronald DePinho Lab
Street address 1901 East Rd
City Houston
State/province Tx
ZIP/Postal code 77054
Country USA
 
Platforms (7)
GPL4910 [Hs35b_P01R] Affymetrix Human Tiling 2.0R Set, Array 1
GPL4911 [Hs35b_P02R] Affymetrix Human Tiling 2.0R Set, Array 2
GPL4912 [Hs35b_P03R] Affymetrix Human Tiling 2.0R Set, Array 3
Samples (63)
GSM272773 ER alpha localization in MCF7 (Whole Genome #1, Replicate #1)
GSM272774 ER alpha localization in MCF7 (Whole Genome #2, Replicate #1)
GSM272775 ER alpha localization in MCF7 (Whole Genome #3, Replicate #1)
Relations
BioProject PRJNA107383

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10800_RAW.tar 1.6 Gb (http)(custom) TAR (of CEL)
Processed data not provided for this record

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