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| Status |
Public on Dec 26, 2018 |
| Title |
Gene expression profile for B6 wild-type and B6 Bgn gene knockout mice (Bgn0/-) with and without fructose treatment by RNA-Seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the effects of fructose on affecting the gene expressions in the hippocampus of male B6 wild-type and Bgn gene knockout (Bgn0/-) mice by RNA-Seq.
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| Overall design |
At 3 months of age, the mice were divided into groups of similar mass and body composition within genotype as follows: wild type with regular drinking water, wild type with fructose (15% w/v) in the drinking water, Bgn0/- with regular drinking water, and Bgn0/- with fructose (15% w/v) in the drinking water for 6 weeks. After collecting phenotypic data, mice were sacrificed and the fresh hippocampus tissues were dissected out, flash frozen, and stored at -70°C for later transcriptome sequencing experiments. All experiments were performed in accordance with the United States National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the University of California at Los Angeles Chancellor’s Animal Research Committee.
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| Contributor(s) |
Meng Q, Yang X |
| Citation(s) |
30291886 |
| |
| Submission date |
Dec 22, 2017 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Xia Yang |
| E-mail(s) |
xyang123@ucla.edu
|
| Organization name |
UCLA
|
| Department |
IBP
|
| Street address |
2000 Terasaki Life Sciences Bldg, 610 Charles E. Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA427306 |
| SRA |
SRP127396 |
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