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| Status |
Public on Dec 27, 2017 |
| Title |
RNA sequencing of hPSC-derived cardiac progenitors and endocardium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We utilized a dual reporting hPSC line that identified cells expressing NKX2.5 and endothelal cells to characterize discrete milestones during cardiac and vascular differentiaiton. Comparing populations that express either or both reporters to human umbilical vein endothelial cells, we document a unique molecular phenotype in hPSC-derived endocardium that points toward an important role for Wnt signaling during vascular specification of cardiac progenitors.
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| Overall design |
Sequencing mRNA from biological triplicate samples of: 1) hPSC derivatives expressing NKX2.5 alone; 2) hPSC derivatives expressing NKX2.5 and endothelial markers; and 3) hPSC derivatives expressing only endothelial markers.
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| |
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| Contributor(s) |
James D |
| Citation(s) |
29217753 |
| |
| Submission date |
Dec 22, 2017 |
| Last update date |
Jul 08, 2019 |
| Contact name |
Daylon James |
| E-mail(s) |
djj2001@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Street address |
515 East 71st Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (9)
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| Relations |
| BioProject |
PRJNA427332 |
| SRA |
SRP127411 |
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