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| Status |
Public on Jan 05, 2018 |
| Title |
Histone variant H3.3-mediated chromatin remodeling is essential for paternal genome activation in mouse preimplantation embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
H3.3-mediated paternal chromatin remodeling is essential for the development of preimplantation embryos and the activation of the paternal genome during embryogenesis.
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| Overall design |
We collected embryos of WT, mH3.3KD and H3.3-addback at 50h after ICSI (8-cell or 16-cell stage). RNA-seq results confirmed excellent knockdown of both H3.3A and H3.3B mRNAs in mH3.3KD and H3.3-addback embryos.
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| |
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| Contributor(s) |
Kong Q, Banaszynski LA |
| Citation(s) |
29358330 |
| |
| Submission date |
Jan 04, 2018 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Xiaolei Zhang |
| E-mail(s) |
zhangxiaolei217@neau.edu.cn
|
| Organization name |
Northeast Agricultural University
|
| Street address |
No.59 Mucai street
|
| City |
Harbin |
| ZIP/Postal code |
150000 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA428516 |
| SRA |
SRP130933 |
