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| Status |
Public on Jun 22, 2019 |
| Title |
Single-cell transcriptomic analysis reveals lineage hierarcies and communications for second heart lineage deployment [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used single-cell RNA-seq to reconstruct differentiation paths of cardiac progenitors in two sequential waves during early heart development. Further analysis identified six major cell types and multiple differentiation trajectories of cardiac progenitors derived from distinct heart fields. We also constructed TF regulatory networks controlling SHF CPC differentiation. Interlineage crosstalk through signaling pathways and chemotactic guidance played a potent role in SHF CPC deployment. The mechanisms regulating SHF CPC migration and differentiation was further confirmed by Nkx2-5 CPC enhancer knock out. Our work provides a cardiac lineage hierarchy and new insights of SHF CPC development.
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| Overall design |
Single cells were derived for scRNA-seq from mice carrying Isl1Cre::Rosa26tdTomato or Nkx2-5Cre::Rosa26tdTomato at E7.75, E8.25, E8.75, E9.25
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| |
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| Contributor(s) |
Xiong H, Luo Y, He A |
| Citation(s) |
31221018 |
| |
| Submission date |
Jan 08, 2018 |
| Last update date |
Jun 22, 2019 |
| Contact name |
Haiqing Xiong |
| E-mail(s) |
haiqingxiong@pku.edu.cn
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| Organization name |
Peking University
|
| Street address |
5 Yiheyuan Road, Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE108963 |
Single-cell transcriptomic analysis reveals lineage hierarcies and communications for second heart lineage deployment |
|
| Relations |
| BioProject |
PRJNA428993 |
| SRA |
SRP128560 |
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