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Series GSE109750 Query DataSets for GSE109750
Status Public on Dec 31, 2019
Title Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the application of RNA-sequencing technology for high-throughput profiling of tumor-associated microglia/macrophage (TAMs). First, we checked the purity of sample by qRT-PCR. We confirmed the high purity of TAMs with rare other cell type contamination. By profiling samples of TAMs purified from five individual medulloblastoma of mouse model, we find that TAMs maintain microglia gene signature even there are decrease of microglia genes and increase of macrophage genes. This study provides the evidence for the application of comprehensive gene profiling towards characterization of cellular origin of cell populations in tumor microenvironment.
 
Overall design Comparing the gene profile between tumor associated microglia/macrophage and normal microglia
Web link https://www.ncbi.nlm.nih.gov/pubmed/31983537
 
Contributor(s) Zong H, Yao M
Citation(s) 31983537
Submission date Jan 28, 2018
Last update date Mar 31, 2020
Contact name Hui Zong
E-mail(s) hz9s@virginia.edu
Phone 4349821956
Organization name University of Virginia
Department Microbiology, immunology and cancer biology
Lab Mouse models of brain cancers
Street address 7046 Jordan Hall, 7th Floor, 1340 JPA
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platforms (2)
GPL9185 Illumina Genome Analyzer (Mus musculus)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM2966313 TAMs 1
GSM2966314 TAMs 2
GSM2966315 TAMs 3
Relations
BioProject PRJNA431915
SRA SRP131618

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109750_Genelist_.txt.gz 2.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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