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Status |
Public on Dec 31, 2019 |
Title |
Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We report the application of RNA-sequencing technology for high-throughput profiling of tumor-associated microglia/macrophage (TAMs). First, we checked the purity of sample by qRT-PCR. We confirmed the high purity of TAMs with rare other cell type contamination. By profiling samples of TAMs purified from five individual medulloblastoma of mouse model, we find that TAMs maintain microglia gene signature even there are decrease of microglia genes and increase of macrophage genes. This study provides the evidence for the application of comprehensive gene profiling towards characterization of cellular origin of cell populations in tumor microenvironment.
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Overall design |
Comparing the gene profile between tumor associated microglia/macrophage and normal microglia
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Web link |
https://www.ncbi.nlm.nih.gov/pubmed/31983537
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Contributor(s) |
Zong H, Yao M |
Citation(s) |
31983537 |
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Submission date |
Jan 28, 2018 |
Last update date |
Mar 31, 2020 |
Contact name |
Hui Zong |
E-mail(s) |
hz9s@virginia.edu
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Phone |
4349821956
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Organization name |
University of Virginia
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Department |
Microbiology, immunology and cancer biology
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Lab |
Mouse models of brain cancers
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Street address |
7046 Jordan Hall, 7th Floor, 1340 JPA
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City |
Charlottesville |
State/province |
VA |
ZIP/Postal code |
22908 |
Country |
USA |
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Platforms (2) |
GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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Samples (8)
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Relations |
BioProject |
PRJNA431915 |
SRA |
SRP131618 |