 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 01, 2018 |
| Title |
Changes in gene expression in human skeletal stem cells transduced with constitutively active Gsα correlates with hallmark histopathological changes seen in fibrous dysplastic bone |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to dissect pathways enchained in skeletal stem/progenitors by Fibrous Dysplasia mutations, we engineered human skeletal stem/progenitors with the mutation and performed transcriptomic analysis. FD mutation profoundly alters the properties of skeletal stem/progenitors by pushing hBMSCs towards formation of disorganized bone with a concomitant lack of fat development. In addition, the mutation created an altered in trans environment that pushed the overall system towards neovascularization, inflammation and osteoclastogenesis.
We used microarrays to detail the global programme of gene expressionc of human skeletal progenitors containing the Gsα activating mutation R201C
|
| |
|
| Overall design |
Human bone marrow stromal cells (hBMSCs) (derived from bone marrow aspirates) from three independent healthy donors were isolated. Lentiviral vectors (LV-GSαR201C and LV-ctr) were generated, produced and titrated. The LV-vector integrated copy number was calculated by Q-PCR as described and was established as ~1 copy of integrated lentiviral sequence per transduced cell. hBMSCs were transduced with LV-GSαR201C and LV-ctr or mock treated.
|
| |
|
| Contributor(s) |
Raimondo D, Remoli C, Astrologo L, Burla R, La Torre M, Verni’ F, Tagliafico E, Corsi A, Robey PG, Riminucci M, Saggio I, Persichetti A, Astrologo L, Del Giudice S, Giannicola G |
| Citation(s) |
31999703 |
| |
| Submission date |
Jan 29, 2018 |
| Last update date |
Feb 10, 2020 |
| Contact name |
domenico raimondo |
| E-mail(s) |
domenico.raimondo@uniroma1.it
|
| Organization name |
Sapienza University of Rome
|
| Department |
Molecular Medicine
|
| Lab |
Stem cells
|
| Street address |
Viale Regina Elena 324
|
| City |
Rome |
| ZIP/Postal code |
00161 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (9)
|
| GSM2970385 |
control cells, biological rep2 |
| GSM2970386 |
mock-treated cells , biological rep2 |
| GSM2970387 |
R201C mutation containing cells, , biological rep2 |
| GSM2970388 |
control cells, biological rep3 |
| GSM2970389 |
mock-treated cells, biological rep3 |
| GSM2970390 |
R201C mutation containing cells, biological rep3 |
|
| Relations |
| BioProject |
PRJNA432079 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109818_RAW.tar |
70.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...