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Series GSE110062 Query DataSets for GSE110062
Status Public on Feb 02, 2021
Title Transcriptional landscape of Myceliophthora thermophila responding to soluble starch and the role of regulator AmyR on polysaccharide degradation
Organism Thermothelomyces thermophilus
Experiment type Expression profiling by high throughput sequencing
Summary Thermophilic fungus Myceliophthora thermophila with great capacity for polysaccharides degradation is attractive to be engineered into a cell factory to produce chemicals and biofuels directly from renewable polysaccharides such as starch. Understanding the molecular mechanism of starch degradation of the fungi would be helpful. To date, there has been no transcriptome analysis on starch in thermophilic fungi. In this study, we performed the transcriptomic profile of M. thermophila responding to soluble starch, and a 342-gene set was identified as “starch regulon”, including the major amylolytic enzyme (Mycth_72393), which was verified thereafter as the most important such hydrolase for starch degradation in this fungus. Moreover, the function of key amylolytic enzyme regulator AmyR in M. thermophila was evaluated by analyzing the performance of its deletion mutant using our CRISPR/Cas9 system, which showed significantly decreased amylase activity and poor growth on starch. Additionally, deletion of amyR led to resistance to carbon catabolite repression (CCR) and enhanced cellulases production. Our study provides an insight into understanding the molecular basis of starch degradation in this thermophilic fungus, and will accelerate the fungal strain rational engineering for starch-based biochemical production.
 
Overall design mRNA profiles of Myceliophthora thermophila cells exposed to carbon starvation, glucose and starch.
 
Contributor(s) Li J, Tian C
Citation(s) 29843921
Submission date Feb 02, 2018
Last update date Sep 16, 2022
Contact name Jingen Li
E-mail(s) li_jg@tib.cas.cn
Phone +86-02284861948
Organization name Tianjin institute of industrial biotechnology,Chinses Academy of Sciences
Street address Xiqi Dao 32, Tianjin Airport Economic Area
City Tianjin
ZIP/Postal code 300308
Country China
 
Platforms (1)
GPL24572 Illumina HiSeq 2000 (Thermothelomyces thermophila)
Samples (6)
GSM2977199 NoCarbon4h_1
GSM2977200 NoCarbon4h_2
GSM2977201 Glucose4h_1
Relations
BioProject PRJNA432703
SRA SRP132068

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Supplementary file Size Download File type/resource
GSE110062_Readcounts.xlsx 1.3 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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