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| Status |
Public on Jul 26, 2018 |
| Title |
Genome-wide maps of STAT1, STAT2 and IRF9 in conjunction with nucleosome occupancy maps at 20 interferon-stimulated gene regions during steady state and after type I interferon stimulation. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The genome-wide distributions of STAT1, STAT2 and IRF9 in mock and interferon alpha (IFN) stimulated human cells was generated using high throughput sequencing to identify ISGF3 genomic occupancy. In combination, the nucleosome occupancy of 20 select IFN-stimulated gene regions were analyzed by direct selection MNase sequencing to determine the nucleosome positions and changes in nucleosome occupancy levels following IFN treatment over time. IFN stimulation resulted in decreased nucleosome positioning strength, with clear disruption over the course of IFN treatment followed by a return to steady state, and prominently at most ISGF3-bound regions.
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| Overall design |
Examination of 3 proteins genome-wide and nucleosome occupancy at 20 interferon-stimulated gene regions during steady state and after type I interferon stimulation in 1 human cell line.
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| |
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| Contributor(s) |
Au-Yeung N, Horvath CM |
| Citation(s) |
30240626 |
| |
| Submission date |
Feb 02, 2018 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Nancy Au-Yeung |
| E-mail(s) |
wauyeung@u.northwestern.edu
|
| Phone |
7202315977
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| Organization name |
Northwestern University
|
| Street address |
2200 Campus Dr
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
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| Platforms (1) |
| GPL16288 |
AB 5500xl Genetic Analyzer (Homo sapiens) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA432700 |
| SRA |
SRP132066 |
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