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Status |
Public on Jun 14, 2018 |
Title |
Run or die in the evolution of de novo microRNAs |
Organisms |
Drosophila erecta; Drosophila melanogaster; Drosophila sechellia; Drosophila simulans |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
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Summary |
MicroRNAs (miRNAs) are a class of small, endogenous RNAs that regulate mRNA expressions post-transcriptionally. To study their expression, we collected and sequenced small RNA libraries in Drosophila species. To survey their regulation divergence, we dissected testes from miR-983 mutant and control flies in two sibling species and performed an RNA-seq analysis.
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Overall design |
Two small RNA samples from D. erecta and D. sechellia testes as well as eight RNA-seq samples from D. melanogaster and D. simulans testes were analyzed. For RNA-seq, each genotype contains two biological replicates.
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Web link |
https://www.biorxiv.org/content/early/2018/06/14/345769?rss=1
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Contributor(s) |
Zhao Y, Yang H, Lin P, Liufu Z, Lu G, Xu J, Tang T, Wen H, Wu C |
Citation missing |
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Submission date |
Feb 02, 2018 |
Last update date |
Jan 23, 2019 |
Contact name |
Yixin Zhao |
E-mail(s) |
yizhao@cshl.edu
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Organization name |
Cold Spring Harbor Laboratory
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Street address |
1 Bungtown Road
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City |
Cold Spring Harbor |
State/province |
NEW YORK |
ZIP/Postal code |
11724 |
Country |
USA |
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Platforms (4)
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GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
GPL13306 |
Illumina HiSeq 2000 (Drosophila simulans) |
GPL19321 |
Illumina HiSeq 2000 (Drosophila erecta) |
GPL20000 |
Illumina HiSeq 2000 (Drosophila sechellia) |
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Samples (10)
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Relations |
BioProject |
PRJNA432727 |
SRA |
SRP132078 |