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Status |
Public on Feb 01, 2019 |
Title |
Transcriptional profiling of rice gene expression changes upon co-infection with pathogens Magnapothe oryzae and Xanthomonas oryzae pv. oryzae that causes rice blast and bacterial blight respectively. |
Organism |
Oryza sativa |
Experiment type |
Expression profiling by array
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Summary |
Yield loss in crop plants due to biotic stresses is a major problem and pyramiding of R genes is often suggested for sustained and durable resistance against target pests. Information is available for single R gene interaction in rice and other crop plants, but not much data is available for multiple R gene interaction against multiple pathogens/pests. In this study we carried out transcriptional analysis of two rice lines introgressed with either R gene against bacterial blight and gall midge or, against bacterial blight and fungal blast. The gene expression changes were investigated through microarray and the expression pattern upon co-infection with multiple pathogens was analyzed to study the synergistic effect of R gene mediated defense responses as well as to explore the possibility of any antagonism between the R genes as defense pathway employed due to R gene mediated resistance varies as per the attacking pathogen/pest.
Keywords: Expression profiling by array
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Overall design |
For the experiment, 25 day old rice plants (Rice line RPNF08 carrying bacterial blight resistance gene Xa21, xa13 and rice blast resistance genes Pi54) were infected with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe oryzae that cause bacterial blight and rice blast respectively. Thereafter, total RNA was isolated from tissue samples that were collected from control (uninfected/mock inoculation) and treated plants (infected with the pathogens) at 24 and 72 hours post inoculation. The RNA sample from the two time points were pooled and used for expression analysis using Affymetrix GeneChip System. Three biological replicates were used for the experiment.
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Contributor(s) |
Maku RV, Divya D, Dass AM, Madhavi RR, Mallikarjuna G, Ramesh P, Madhavi M, Prasad SM, Sundaram RM, Laha GS, Padmakumari AP, Patel HK, Bentur JS, Sonti RV |
Citation missing |
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Submission date |
Feb 02, 2018 |
Last update date |
Feb 02, 2019 |
Contact name |
Ramesh V. Sonti |
E-mail(s) |
sonti@ccmb.res.in
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Organization name |
Centre for cellular and molecular biology (CCMB)
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Department |
Plant Microbe Interactions
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Street address |
Uppal Road
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City |
Hyderabad |
State/province |
Andhra Pradesh |
ZIP/Postal code |
500007 |
Country |
India |
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Platforms (1) |
GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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Samples (6)
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GSM2977446 |
Magnaporthe oryzae and Xantomonas oryzae pv. oryzae infected rice plants, bio rep 1 |
GSM2977447 |
Magnaporthe oryzae and Xantomonas oryzae pv. oryzae infected rice plants, bio rep 2 |
GSM2977448 |
Magnaporthe oryzae and Xantomonas oryzae pv. oryzae infected rice plants, bio rep 3 |
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Relations |
BioProject |
PRJNA432728 |