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Series GSE110088 Query DataSets for GSE110088
Status Public on Feb 01, 2019
Title Transcriptional profiling of rice gene expression changes upon co-infection with pathogens Magnapothe oryzae and Xanthomonas oryzae pv. oryzae that causes rice blast and bacterial blight respectively.
Organism Oryza sativa
Experiment type Expression profiling by array
Summary Yield loss in crop plants due to biotic stresses is a major problem and pyramiding of R genes is often suggested for sustained and durable resistance against target pests. Information is available for single R gene interaction in rice and other crop plants, but not much data is available for multiple R gene interaction against multiple pathogens/pests. In this study we carried out transcriptional analysis of two rice lines introgressed with either R gene against bacterial blight and gall midge or, against bacterial blight and fungal blast. The gene expression changes were investigated through microarray and the expression pattern upon co-infection with multiple pathogens was analyzed to study the synergistic effect of R gene mediated defense responses as well as to explore the possibility of any antagonism between the R genes as defense pathway employed due to R gene mediated resistance varies as per the attacking pathogen/pest.

Keywords: Expression profiling by array
 
Overall design For the experiment, 25 day old rice plants (Rice line RPNF08 carrying bacterial blight resistance gene Xa21, xa13 and rice blast resistance genes Pi54) were infected with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe oryzae that cause bacterial blight and rice blast respectively. Thereafter, total RNA was isolated from tissue samples that were collected from control (uninfected/mock inoculation) and treated plants (infected with the pathogens) at 24 and 72 hours post inoculation. The RNA sample from the two time points were pooled and used for expression analysis using Affymetrix GeneChip System. Three biological replicates were used for the experiment.
 
Contributor(s) Maku RV, Divya D, Dass AM, Madhavi RR, Mallikarjuna G, Ramesh P, Madhavi M, Prasad SM, Sundaram RM, Laha GS, Padmakumari AP, Patel HK, Bentur JS, Sonti RV
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Submission date Feb 02, 2018
Last update date Feb 02, 2019
Contact name Ramesh V. Sonti
E-mail(s) sonti@ccmb.res.in
Organization name Centre for cellular and molecular biology (CCMB)
Department Plant Microbe Interactions
Street address Uppal Road
City Hyderabad
State/province Andhra Pradesh
ZIP/Postal code 500007
Country India
 
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (6)
GSM2977443 Uninfected rice plants, bio rep 1
GSM2977444 Uninfected rice plants, bio rep 2
GSM2977445 Uninfected rice plants, bio rep 3
Relations
BioProject PRJNA432728

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110088_RAW.tar 26.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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