|
| Status |
Public on Oct 09, 2018 |
| Title |
Analysis of LOV-FPs-mediated stress response in E. coli. |
| Platform organisms |
Gluconobacter oxydans; Escherichia coli; Corynebacterium glutamicum; Bacillus subtilis subsp. subtilis str. 168 |
| Sample organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by array
|
| Summary |
Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed the ROS-induced stress responses of the two variants DsFbFP M49I and Pp2FbFP, exhibiting preferential photosensitization of superoxide and singlet oxygen, respectively, in E.coli.
|
| |
|
| Overall design |
To analyze differences in global oxidative stress response in E. coli that were induced by Pp2FbFP and DsFbFP M49I, a DNA microarray-based analysis was conducted. E. coli cells expressing the respective photosensitizers were cultivated in LB media. LOV-FP expression was induced by addition of 0.4 mM IPTG during the logarithmic growth phase (~OD580 = 0.7). Three hours after induction, cultures were divided into two test cultures with a volume of 25 ml in 500 ml Erlenmeyer flaks and an OD580 of 0.5. For light treatment one of these cultures was placed between two blue-light emitting LED-panels, whereas the second culture was kept in the dark. Detailed transcriptome analyses of light-induced stress-response based on independent biological triplicates were finally carried out under best illumination conditions (i.e. Pp2FbFP: 5 min, DsFbFP M49I: 15 min).
|
| |
|
| Contributor(s) |
Endres S, Polen T, Drepper T |
| Citation(s) |
30301917 |
| |
| Submission date |
Feb 05, 2018 |
| Last update date |
Oct 17, 2018 |
| Contact name |
Tino Polen |
| E-mail(s) |
t.polen@fz-juelich.de
|
| Organization name |
Forschungszentrum Jülich GmbH
|
| Department |
IBG-1: Biotechnology
|
| Street address |
Leo Brandt Str.
|
| City |
Juelich |
| State/province |
NRW |
| ZIP/Postal code |
52425 |
| Country |
Germany |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA433001 |
Less...
More...