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| Status |
Public on Feb 07, 2018 |
| Title |
Neuro-glial crosstalk regulates microglia activation and control of viral encephalitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study it was possible to compare the behaviour of different myeloid cell populations upon VSV infection.
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| Overall design |
Non-activated microglia (Ly6G-CD45lowCD11b+Tomato+ cells) and activated microglia (Ly6G-CD45highCD11b+Tomato+ cells) from olfactory bulb and infiltrating monocytes (Ly6G-CD45highCD11bhigh-Tomato-) were FACS-sorted from Cx3cr1CreER:R26-Tomato mice in which all resident macrophages express the endogenous tomato signal while peripheral immune cells do not. The mRNA from the different populations was extracted and sequenced on Illumina HiSeq RNA-Seq. Three replicates per condition were used. As a control (reference samples) were used three replicates from non-activated microglia from non-infected mice (CCE9701-CCE9703). All the other samples are coming from VSV-infected mice.
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| Contributor(s) |
Chhatbar C, Jordao M, Prinz M, Kalinke U |
| Citation(s) |
30282022 |
| |
| Submission date |
Feb 06, 2018 |
| Last update date |
Apr 06, 2020 |
| Contact name |
Marta Joana Costa Jordão |
| Organization name |
Uniklinikum Freiburg
|
| Department |
Neuropathology
|
| Street address |
Breisacher strasse 64
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA433097 |
| SRA |
SRP132259 |
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