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| Status |
Public on Feb 08, 2018 |
| Title |
Sulfamethoxazole – Trimethoprim represses csgD but 2 maintains virulence genes at 30°C in a clinical 3 Escherichia coli O157:H7 isolate |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The high frequency of prophage insertions in the mlrA gene of clinical serotype O157:H7 isolates renders such strains deficient in csgD-dependent biofilm formation but prophage induction may restore certain mlrA properties. In this study we used transcriptomics to study the effect of high and low sulfamethoxazole–trimethoprim (SMX-TM) concentrations on prophage induction, biofilm regulation, and virulence gene expression in strain PA20 under environmental conditions following five-hour and 12-hour exposures. SMX-TM at a sub-lethal concentration induced strong RecA expression resulting in concentration- and time-dependent major transcriptional shifts with emphasis on up-regulation of genes within horizontally-transferred chromosomal regions (HTR). Neither high or low levels of SMX-TM stimulated csgD expression at either time point, but both levels resulted in slight suppression. Full expression of Ler-dependent genes paralleled expression of group 1 pch homologues in the presence of high glrA. Finally, stx2 expression, which is strongly dependent on prophage induction, was enhanced at 12 hours but suppressed at five hours, in spite of early SOS initiation by the high SMX-TM concentration. Our findings indicate that, similar to host conditions, exposure to environmental conditions increased the expression of virulence genes in a clinical isolate but genes involved in the protective biofilm response were suppressed.
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| Overall design |
We evaluated mRNA expression profile of 2 time points and 3 sulfamethoxazole–trimethoprim (SMX-TM) concentrations
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| Contributor(s) |
Uhlich GA, Andreozzi E, Cottrell BJ, Reichenberger ER, Zhang X, Paoli GC |
| Citation(s) |
29718957 |
| BioProject |
PRJNA294158 |
| |
| Submission date |
Feb 07, 2018 |
| Last update date |
Jan 07, 2019 |
| Contact name |
Gaylen C Ulrich |
| E-mail(s) |
George.Paoli@ars.usda.gov
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| Organization name |
USDA/ARS/Eastern Regional Research Center
|
| Street address |
600 East Mermaid Lane
|
| City |
Wyndmoor |
| State/province |
PA |
| ZIP/Postal code |
19038 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18133 |
Illumina HiSeq 2500 (Escherichia coli) |
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| Samples (18)
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| Relations |
| SRA |
SRP063289 |
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