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| Status |
Public on Apr 16, 2018 |
| Title |
Characterization of the trehalose utilization operon in Streptococcus mutans reveals that the TreR transcriptional regulator is involved in stress response pathways and toxin production |
| Organism |
Streptococcus mutans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Streptococcus mutans, the organism most frequently associated with the development of dental caries, is able to utilize a diverse array of carbohydrates for energy metabolism. One such molecule is trehalose, a disaccharide common in human foods, which has recently been implicated in enhancing the virulence of epidemic strains of the pathogen, Clostridium difficile. In this study, deletion mutants of all three genes in the putative S. mutans trehalose utilization operon were characterized and shown to be required for wild-type levels of growth when trehalose was the only carbohydrate source provided. Interestingly, the TreR transcriptional regulator appeared to be critical for responding to oxidative stress, and for mounting a protective stress tolerance response following growth at moderately acidic pH. RNAseq of a treR deletion mutant suggested that in S. mutans, TreR acts as a trehalose-sensing activator of transcription of the tre operon, rather than a repressor, as described in other species. In addition, deletion of treR caused the down-regulation of a number of genes involved in genetic competence and bacteriocin production, supporting results of a recent study linking trehalose and the S. mutans competence pathways. Finally, deletion of treR compromised the ability of S. mutans to inhibit the growth of the competing species, Streptococcus gordonii and Lactococcus lactis. Taken together, this study solidifies the role of the S. mutans tre operon in trehalose utilization and suggests novel functions for the TreR regulator, including roles in stress response and competitive fitness.
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| Overall design |
Transcriptional profiling was performed using RNA extracted from cultures of S. mutans parent strain UA159 and the ∆treR derivative grown in tryptone-yeast extract medium supplemented with either glucose, galactose or trehalose.
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| Contributor(s) |
Baker JL, Lindsay EL, Faustoferri RC, To TT, Hendrickson EL, He X, Shi W, McLean JS, Quivey RG Jr |
| Citation(s) |
29632089 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| T90 DE021985 |
Training Program in Oral Science |
UNIVERSITY OF ROCHESTER |
Robert G Quivey |
| F32 DE026947 |
Understanding S. Mutans Pathogenesis in a Social and Ecological Setting |
J. Craig Venter Institute |
Jonathon Baker |
| R01 DE020102 |
Making a quantum leap in plaque research with modern sciences |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Xuesong He |
| R01 DE020102 |
Making a quantum leap in plaque research with modern sciences |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Jeffrey Scott McLean |
| R01 DE020102 |
Making a quantum leap in plaque research with modern sciences |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Wenyuan Shi |
| R01 DE026186 |
Studying the Protective Effects of Normal Oral Flora |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Xuesong He |
| R01 DE026186 |
Studying the Protective Effects of Normal Oral Flora |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Jeffrey Scott McLean |
| R01 DE026186 |
Studying the Protective Effects of Normal Oral Flora |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Wenyuan Shi |
| R01 DE013683 |
Oxidative Stress Responses in Oral Streptococci |
UNIVERSITY OF ROCHESTER |
Robert G Quivey |
| R01 DE017425 |
Fitness profiling of the Streptococcus mutans genome |
UNIVERSITY OF ROCHESTER |
Robert G Quivey |
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| Submission date |
Feb 07, 2018 |
| Last update date |
Apr 16, 2018 |
| Contact name |
Roberta C Faustoferri |
| E-mail(s) |
roberta_faustoferri@urmc.rochester.edu
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| Organization name |
University of Rochester Medical Center
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| Department |
Center for Oral Biology
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| Lab |
Dr. Robert Quivey
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| Street address |
601 Elmwood Ave. Box 611
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL24591 |
NextSeq 550 (Streptococcus mutans) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA433360 |
| SRA |
SRP132367 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110275_DeSeq2_treR.txt.gz |
404.8 Kb |
(ftp)(http) |
TXT |
| GSE110275_treR_RNAseq_Processed_Data_file.xlsx |
1.9 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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