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Status |
Public on Dec 03, 2009 |
Title |
Duck versus Chicken |
Platform organisms |
Gallus gallus; Homo sapiens; Mus musculus |
Sample organisms |
Anas platyrhynchos; Gallus gallus |
Experiment type |
Expression profiling by array
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Summary |
Avian beaks show extreme species-specific variability in morphology, though they develop from the same primordial structures. In both humans and birds, cranial neural crest cells are the primary source of mesenchyme for the frontonasal prominence; previous work has shown that these cells contain molecular information that regulate species-specific facial variation. To determine the molecular basis of avian craniofacial patterning, we have gene expression profiled micro-dissected cranial neural crest cells from the frontonasal prominence of three bird species (chickens, quails, and ducks) during embryonic development. These changes in gene expression were measured on a custom built, cross-species, long oligonucleotide microarray that interrogates the vast majority of transcription factor (TF) genes plus a wide variety of signaling pathways. Samples were isolated at two developmental stages, before (Hamburger Hamilton stage [HH] 20) and after (HH25) morphological distinctions between the species are evident. Keywords: cross-species comparison
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Overall design |
Frontonasal mesenchymal cells were micro-dissected two developmental stages, before (Hamburger Hamilton stage [HH] 20) and after (HH25) morphological distinctions between the species are evident. For each sample, mesenchyme from 40 embryos was pooled. For each stage-matched comparison between duck and chicken, experiments include technical replicates as well as dye-switches for a total of 14 microarrays.
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Contributor(s) |
Powder KE, Brugmann SA, Helms JA, Lovett M |
Citation(s) |
20015954 |
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Submission date |
Apr 02, 2008 |
Last update date |
Jan 18, 2013 |
Contact name |
Kara Powder |
E-mail(s) |
kara.powder@gmail.com
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Organization name |
Washington University in St Louis
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Department |
Genetics
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Lab |
Lovett
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Street address |
4566 Scott Ave
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City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63108 |
Country |
USA |
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Platforms (1) |
GPL6648 |
Lovett/WUSTL 0.7k TFarray v3 |
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Samples (14)
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This SubSeries is part of SuperSeries: |
GSE11099 |
Molecular basis of species-specific variation in beak morphology |
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Relations |
BioProject |
PRJNA108803 |