NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE110352 Query DataSets for GSE110352
Status Public on Aug 23, 2018
Title Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin organisation is disrupted genome wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naïve histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is copied during DNA replication or the epigenomeis perturbed. Here we develop Chromatin Occupancy after Replication, ChOR-seq, a technology that combines chromatin immunopreciptation of histone marks and purification of newly replicated DNA by streptavidin pull-down followed by next generation sequencing. We use this technology to address propagation of epigenetic states across cell division and reveal that accurate recycling of modified parental histones ensures that positional information of histone marks is faithfully reproduced on daughter strands and inherited to daughter cells.
 
Overall design Analysis of H3K27me3 and total H3 distribution and abundance after replication and across the cell cycle by ChOR-seq in HeLa S3 cells. All experiments were done in duplicates. Replicated chromatin was labelled in mid S. Chromatin of Drosophila melanogater S2 cells were used as spike-in for normalization purpose.
 
Contributor(s) Reverón-Gómez N, González-Aguilera C, Stewart-Morgan KR, Groth A
Citation(s) 30146316
Submission date Feb 08, 2018
Last update date Mar 26, 2019
Contact name Anja Groth
E-mail(s) anja.groth@cpr.ku.dk
Organization name Novo Nordisk Foundation Center for Protein Research
Street address Blegdamsvej 3B
City Copenhagen
ZIP/Postal code 2200
Country Denmark
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (24)
GSM2988386 H3K27me3_ChIP-seq_Parental_rep1
GSM2988387 H3K27me3_ChOR-seq_T0_rep1
GSM2988388 H3K27me3_ChOR-seq_T4_rep1
This SubSeries is part of SuperSeries:
GSE110354 Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication
Relations
BioProject PRJNA433528
SRA SRP132492

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110352_RAW.tar 799.9 Mb (http)(custom) TAR (of BED, BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap