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Status |
Public on Feb 13, 2019 |
Title |
The bovine alveolar macrophage (bAM) DNA methylome is resilient to infection with Mycobacterium bovis |
Organism |
Bos taurus |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
Whole genome bisulfite sequencing (WGBS) was used to assess the effect of M. bovis infection on the bAM DNA methylome (5-methylcytosine). The methylomes of bAM infected with M. bovis (n = 8) were compared to those of non-infected control bAM (n = 8) at 24 hours post-infection (hpi). No differences in DNA methylation (CpG or non-CpG) were observed between control and infected bAM. Analysis of DNA methylation at proximal promoter regions uncovered >250 genes harbouring intermediately methylated (IM) promoters (average methylation = 33–66%). Gene ontology (GO) analysis, focusing on genes with low, intermediate or highly methylated promoters, revealed that genes with IM promoters were enriched for immune-related GO categories; this enrichment was not observed for genes in the high or low methylation groups. Targeted analysis of two non-imprinted genes in the IM category, C1QB and IL2RA, confirmed the WGBS observation. This study is the first in cattle to examine genome-wide DNA methylation at single nucleotide resolution in an important bovine cellular host-pathogen interaction model and provides evidence for intermediate promoter methylation in bAM.
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Overall design |
Examination of CpG methylation in control and Mycobacterium bovis infected bovine macrophages, 24 hours post infection
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Contributor(s) |
O'Doherty AM, Rue-Albrecht KC, Magee DA, Ahting S, Irwin RE, Hall TJ, Browne JA, Nalpas NC, Walsh CP, Gordon SV, Wojewodzic MW, MacHugh DE |
Citation(s) |
- O'Doherty AM, Rue-Albrecht KC, Magee DA, Ahting S et al. The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis. Sci Rep 2019 Feb 6;9(1):1510. PMID: 30728374
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Submission date |
Feb 09, 2018 |
Last update date |
Feb 13, 2019 |
Contact name |
Thomas Hall |
E-mail(s) |
tjhall1688@gmail.com
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Phone |
857168930
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Organization name |
UCD
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Department |
Vet sciences
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Street address |
2, Taney Grove
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City |
Dublin |
State/province |
Select One |
ZIP/Postal code |
D14 KW14 |
Country |
Ireland |
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Platforms (1) |
GPL19172 |
Illumina HiSeq 2500 (Bos taurus) |
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Samples (16)
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Relations |
BioProject |
PRJNA433673 |
SRA |
SRP132590 |
Supplementary file |
Size |
Download |
File type/resource |
GSE110412_RAW.tar |
3.4 Gb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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