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Series GSE11044 Query DataSets for GSE11044
Status Public on Apr 29, 2008
Title Differential H3K4 methylation identifies developmentally poised hematopoietic genes
Organism Mus musculus
Experiment type Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
Summary We assess the concordance of histone H3 lysine 4 dimethylation (H3K4me2) and trimethylation (H3K4me3) on a genome-wide scale in erythroid development by analyzing pluripotent, multipotential and unipotent cell types. Although H3K4me2 and H3K4me3 are concordant at most genes, multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent, highly enriched in lineage-specific hematopoietic genes, and uniquely susceptible to differentiation-induced H3K4 demethylation. Self-renewing embryonic stem cells, which restrict H3K4 methylation to genes that contain CpG islands (CGIs), lack H3K4me2+/me3- genes. These data reveal distinct epigenetic regulation of CGI and non-CGI genes during development and indicate an interactive relationship between DNA sequence and differential H3K4 methylation in lineage-specific differentiation.
Keywords: comparison of different cell lines
 
Overall design Multipotential and erythroid-differentiated hematopoietic cells were compared. Expression was measured using Affymetrix microarrays. H3K4me2 and H3K4me3 enrichment was assessed using Agilent tiling arrays.
 
Contributor(s) Orford K, Kharchenko PV
Citation(s) 18477461
Submission date Apr 03, 2008
Last update date Apr 02, 2024
Contact name Peter Kharchenko
Organization name Harvard Medical School
Department DBMI
Lab Kharchenko
Street address 10 Shattuck St.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (3)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL6691 Agilent-014928 Mouse Promoter tiling array set. Microarray 1 of 2
GPL6692 Agilent-014929 Mouse Promoter tiling array set. Microarray 2 of 2
Samples (22)
GSM279054 U-EML replicate 1
GSM279055 U-EML replicate 2
GSM279056 U-EML replicate 3
Relations
BioProject PRJNA107025

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11044_RAW.tar 1.1 Gb (http)(custom) TAR (of CEL, TXT)
Processed data included within Sample table

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