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| Status |
Public on Oct 01, 2019 |
| Title |
RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected uterine smooth muscle cells (USMC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used high throughput sequencing to analyze the gene expression profiling of human uterine smooth muscle cells (USMC). By comparing the gene expression profiling of USMC with or without H19 knockdown, 215 genes were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 228 genes were found at least 1.5-fold down-regulated in siH19 over siCon cells.
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| Overall design |
USMC cells were transfected with either control siRNA or siH19. 48h after transfection, total RNA was extracted for library preparation and RNA-seq analysis to compare gene expression profiles between siCon and siH19 cells.
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| Contributor(s) |
Zhang N, Carmichael GG, Huang Y |
| Citation(s) |
31089260, 32374060 |
| |
| Submission date |
Feb 13, 2018 |
| Last update date |
Jul 20, 2020 |
| Contact name |
Ying Jiang |
| E-mail(s) |
ying.jiang@yale.edu
|
| Organization name |
Department of Obstetrics, Gynecology & Reproductive Sciences
|
| Street address |
310 Cedar Street
|
| City |
New Haven |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA433983 |
| SRA |
SRP132803 |
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