|
| Status |
Public on Jul 26, 2018 |
| Title |
Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
Other
|
| Summary |
Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAprotect. Gene expression analysis was performed against clone- and passage-matched ground control samples.
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| |
|
| Overall design |
qPCR gene expression profiling. Human CPCs from three neonates and three adults were individually cultured on the ground or aboard the ISS. Three measurements were taken per sample. All measurements are included in the supplementary files.
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| |
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| Contributor(s) |
Baio J, Kearns-Jonker M, Martinez AF, Silva IS, Hoehn CV, Countryman S, Bailey L, Hasaniya N, Pecaut MJ |
| Citation(s) |
30062101 |
| |
| Submission date |
Feb 13, 2018 |
| Last update date |
Nov 04, 2018 |
| Contact name |
Jonathan Baio |
| E-mail(s) |
jbaio@llu.edu
|
| Organization name |
Loma Linda University School of Medicine
|
| Department |
Pathology and Human Anatomy
|
| Lab |
Kearns-Jonker
|
| Street address |
24941 Stewart St, Room 326
|
| City |
Loma Linda |
| State/province |
CA |
| ZIP/Postal code |
92354 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (12)
|
| GSM3001077 |
Neonatal Clone 1_conventionalRT-PCR_ground_control |
| GSM3001078 |
Neonatal Clone 1_conventionalRT-PCR_ISS-cultured |
| GSM3001079 |
Neonatal Clone 2_conventionalRT-PCR_ground_control |
| GSM3001080 |
Neonatal Clone 2_conventionalRT-PCR_ISS-cultured |
| GSM3001081 |
Neonatal Clone 3_conventionalRT-PCR_ground_control |
| GSM3001082 |
Neonatal Clone 3_conventionalRT-PCR_ISS-cultured |
| GSM3001083 |
Adult Clone 1_conventionalRT-PCR_ground_control |
| GSM3001084 |
Adult Clone 1_conventionalRT-PCR_ISS-cultured |
| GSM3001085 |
Adult Clone 2_conventionalRT-PCR_ground_control |
| GSM3001086 |
Adult Clone 2_conventionalRT-PCR_ISS-cultured |
| GSM3001087 |
Adult Clone 3_conventionalRT-PCR_ground_control |
| GSM3001088 |
Adult Clone 3_conventionalRT-PCR_ISS-cultured |
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| This SubSeries is part of SuperSeries: |
| GSE110563 |
Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station |
|
| Relations |
| BioProject |
PRJNA434039 |
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