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Series GSE110804 Query DataSets for GSE110804
Status Public on Feb 13, 2019
Title RNA sequencing reveals RNA binding protein HuR/ELAVL1 regulates extracellular matrix gene expression in nucleus pulposus cells of the intervertebral disc
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary We used RNA sequencing to identify transcripts that were controlled by the RNA binding factor HuR/ELAVL1 in nucleus pulposus cells of the intervertebral disc.
Overall design Nucleus pulposus cells were isolated from rat intervertebral discs and cultured under hypoxic conditions. Cells were transduced with either control (MISSION PLKO.1 non-target ShRNA) or HuR-specific shRNA (ShHuR). mRNA was isolated using Rneasy mini columns (Qiagen), treated with DNase I, and RNA sequencing was performed. n=4 for each experimental group.
Contributor(s) Risbud MV, Pan H, Johnson ZI
Citation(s) 30092282
Submission date Feb 19, 2018
Last update date Feb 13, 2019
Contact name Makarand Risbud
Phone (215) 955-1063
Organization name Thomas Jefferson University
Department Orthopaedic Surgery
Lab 511 College Bldg.
Street address 1015 Walnut St.
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (8)
GSM3017014 NP_control 1
GSM3017015 NP_control 2
GSM3017016 NP_control 3
BioProject PRJNA434587
SRA SRP133075

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Supplementary file Size Download File type/resource
GSE110804_ShCTR_Hx_vs_ShHuR_Hx.xlsx 3.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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