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Series GSE110989 Query DataSets for GSE110989
Status Public on Dec 31, 2020
Title Analysis of gene expression in co-culture of PBMCs with colon cancer cell lines by High-Throughput Sequencing
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary (Objectives) The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on colon cancer cell lines.
(Method) Transcription profiles of PBMCs (control) and PBMCs co-cultured with colon cancer cell lines (treatment) were generated by deep sequencing, in triplicate for SW480 and duplicate for HT29, using Illumina HiSeq 4000.
(Results and conclusion) Transcriptome analysis of PBMCs has shown the gene expression changed when co-cultured with colon cancer cell lines. The result revealed significantly up- and down-regulated genes.
 
Overall design Transcription profiles of PBMC (control) and PBMC co-cultured with colon cancer cell lines (treatment) were generated by deep sequencing, in triplicate for SW480 and duplicate for HT29, using Illumina HiSeq 4000.
 
Contributor(s) Boonsongserm P, Kitkumthorn N, Mutirangura A
Citation(s) 31452782
Submission date Feb 22, 2018
Last update date Dec 31, 2020
Contact name Nakarin Kitkumthorn
E-mail(s) nakarinkitkumthorn@gmail.com
Organization name Mahidol University
Department Oral Biology
Street address Yothee road
City Bangkok
ZIP/Postal code 10400
Country Thailand
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (10)
GSM3019770 RCF1: Control Female rep1
GSM3019771 RCF3: Control Female rep2
GSM3019772 RCM1: Control Male rep1
Relations
BioProject PRJNA435504
SRA SRP133271

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110989_control_vs_treatment_HT29.txt.gz 321.7 Kb (ftp)(http) TXT
GSE110989_control_vs_treatment_SW480.txt.gz 452.5 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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