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| Status |
Public on Sep 05, 2018 |
| Title |
Dietary lipids modulate Notch signaling and influence adult intestinal development and metabolism in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We mapped about 25 million reads per sample to the Drosophila melanogaster genome (dm6). We confirmed that sterol processing genes are selectively expressed in the posterior midgut. Modifying sterol metabolism in the diet or with Hr96 mutants resulted in significant changes in metabolism transcripts, hormones expressed in enteroendocrine cells, and Notch pathway related genes.
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| Overall design |
Anterior and posterior midgut mRNA profiles of wild type (y[1]w[1) flies fed a control, lipid-depleted, and high cholesterol (4x control levels) diet, and Hr96[1] and Hr96[2x] mutants. Each sample was sequenced in triplicate using Illuman NextGen sequencing.
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| Contributor(s) |
Obniski R, Sieber M, Spradling AC |
| Citation(s) |
30220569 |
| |
| Submission date |
Feb 23, 2018 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Allan C Spradling |
| E-mail(s) |
spradling@ciwemb.edu
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| Phone |
410 246-3015
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| Organization name |
Carnegie Institution/HHMI
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| Department |
Embryology
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| Street address |
3520 San Martin Dr.
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21218 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA435728 |
| SRA |
SRP133382 |
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