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| Status |
Public on Feb 05, 2020 |
| Title |
DNA:cytoplasm ratio defines an upper limit to cell size [array] |
| Platform organism |
Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae W303 |
| Experiment type |
Expression profiling by array
|
| Summary |
Cell size varies greatly between cell types, yet within a specific cell type and growth condition, cell size is narrowly distributed. Why maintenance of a cell-type specific cell size is important is not understood. Here we show that growing beyond a certain size has wide-ranging effects on cell physiology. Large cells are defective in gene induction, cell cycle progression and cell signaling. We further show that these defects are caused by the inability of large cells to scale nucleic acid and protein biosynthesis in accordance with cell volume increase, which effectively leads to cytoplasm dilution. Finally, we determine why nucleic acid and protein biosynthesis do not scale with cell volume beyond a certain critical size. DNA becomes limiting. We conclude that the correct DNA to cytoplasm ratio is vital for many perhaps all cellular functions and that the range where this ratio supports optimal cell function is remarkably narrow.
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| Overall design |
Two-color microarray experiment comparing gene expression of S. cerevisiae cells of different cell size before and after 40 minutes of alpha factor exposure.
The cell sizes are:
WT: 50 fL
2h: 250 fL
6h: 815 fL
6h CHX: 325 fL
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| |
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| Contributor(s) |
Neurohr GE, Amon A |
| Citation(s) |
30739799 |
| |
| Submission date |
Feb 23, 2018 |
| Last update date |
Feb 07, 2020 |
| Contact name |
Charles Arthur Whittaker |
| E-mail(s) |
charliew@mit.edu
|
| Organization name |
Koch Institute
|
| Street address |
77 Mass Ave 76-189
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02152 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9825 |
Agilent-016322 Yeast (V2) Gene Expression 8x15K Microarray (Feature Number version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE111076 |
DNA:cytoplasm ratio defines an upper limit to cell size |
|
| Relations |
| BioProject |
PRJNA435760 |
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