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| Status |
Public on Feb 25, 2021 |
| Title |
Illumina miRNA sequencing revealed Citrus adaptation to long-term B-toxicity through modulation of lateral root development by miR319 |
| Organisms |
Citrus sinensis; Citrus maxima |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We report the application of Illumina sequencing for high-throughput profiling of miRNA in citrus root responded to long-term boron toxicity. We find miR319 is involved in citrus adapation to long-term boron toxicity via targeting a MYB gene, Ciclev10000756m.g.v1.0, which is homologus with several MYBs that modulate lateral root development in Arabidopsis.
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| Overall design |
Citrus sinensis and C. grandis seedlings were sand cultured and treated with different levels of H3BO3 for 15 weeks. At the end of B treatments, root tips were used for total RNA extraction. small RNA libraries were then constructed and sequenced with an Illumina HiSeq 2500.
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| Contributor(s) |
Huang J, Chen L |
| Citation missing |
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| |
| Submission date |
Feb 26, 2018 |
| Last update date |
Feb 25, 2021 |
| Contact name |
Jing-Hao Huang |
| E-mail(s) |
jhuang1982@126.com
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| Phone |
+8613675012724
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| Organization name |
Fujian Academy of Agricultural Sciences
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| Department |
Pomological Institute
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| Street address |
Wusi Road
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| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350013 |
| Country |
China |
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| Platforms (2) |
| GPL21081 |
Illumina HiSeq 2500 (Citrus maxima) |
| GPL21082 |
Illumina HiSeq 2500 (Citrus sinensis) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA435986 |
| SRA |
SRP133492 |
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