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Status |
Public on May 20, 2019 |
Title |
Whole transcriptomic analysis of zebrafish embryos of dyrk1aakrb1, dyrk1aa knock out mutant and Wild Type (WT) (+/+) in the background of Tg(kdrl:egfp) |
Organism |
Danio rerio |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: The goals of this experiments are to analyze the transcriptomic change in dyrk1aakrb1 mutants compared with WT embryos by mRNA-seq technique. Methods: Whole mRNA profiles of 48 hpf WT(+/+) and dyrk1aakrb1 mutants zebrafish embryos were generated by mRNA-seq techinque, in duplicate, using HiSeq 2500 (Illumina, Inc., USA). Results: This analysis identified 222 transcripts as differentially regulated genes (DEG), of which 101 were up-regulated and 121 were down-regulated in dyrk1aakrb1 mutant (more than 2 fold and less than 0.5 fold respectively,; p value< 0.05).
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Overall design |
Whole transcriptomic analysis of zebrafish embryos of dyrk1aakrb1, dyrk1aa knock out mutant and WT (+/+) in the background of Tg(kdrl:egfp) at 48 hpf.
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Contributor(s) |
Cho H, Lee J, Kim JH, Kim SY |
Citation(s) |
31043432 |
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Submission date |
Feb 28, 2018 |
Last update date |
Jun 20, 2019 |
Contact name |
Jong Hwan Kim |
E-mail(s) |
kkjjhhk@kribb.re.kr
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Phone |
0821093299557
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Organization name |
Korea Research Institute of Bioscience and Biotechnology
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Department |
Genome structure research center
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Street address |
125 Gwahak-ro, Yuseong-gu
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City |
Daejeon |
ZIP/Postal code |
ASI|KR|KS015|DAEJEON |
Country |
South Korea |
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Platforms (1) |
GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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Samples (4)
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Relations |
BioProject |
PRJNA436393 |
SRA |
SRP133688 |