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| Status |
Public on Jun 21, 2018 |
| Title |
Prior activity of olfactory receptor neurons is required for proper sensory processing and behavior in Drosophila larvae |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Animal responses to their environment rely on activation of sensory neurons by external stimuli. In many sensory systems, however, neurons display basal activity prior to the external stimuli. This prior activity is thought to modulate neural functions, yet its impact on animal behavior remains elusive. Here, we reveal a potential role for prior activity in olfactory receptor neurons (ORNs) in shaping larval olfactory behavior. We show that prior activity in larval ORNs is mediated by the olfactory receptor complex (OR complex). Mutations of Orco, an odorant co-receptor required for OR complex function, cause reduced attractive behavior in response to optogenetic activation of ORNs. Calcium imaging reveals that Orco mutant ORNs fully respond to optogenetic stimulation but exhibit altered temporal patterns of neural responses. These findings together suggest a critical role for prior activity in information processing upon ORN activation in Drosophila larvae, which in turn contributes to olfactory behavior control.
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| Overall design |
Four control and five orco mutant samples with 20 dorsal organs each were isolated and subjected to mRNA-Seq analysis
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| Contributor(s) |
Utashiro N, Williams CR, Parrish JZ, Emoto K |
| Citation(s) |
29872087 |
| |
| Submission date |
Mar 02, 2018 |
| Last update date |
Aug 20, 2018 |
| Contact name |
Jay Parrish |
| E-mail(s) |
jzp2@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Department of Biology
|
| Lab |
Parrish Lab
|
| Street address |
Box 351800
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA436648 |
| SRA |
SRP133822 |
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