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Series GSE11149 Query DataSets for GSE11149
Status Public on May 06, 2008
Title Cranial neural crest cells of E11.5 wild type and FAK mutants (valle-affy-mouse-480721)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Cranial neural crest cells (NCC) give rise to the majority of the cartilage, bone, connective tissue, and sensory ganglia in the head, and also participate in the remodeling of the cardiac outflow tract and pharyngeal arch arteries during cardiovascular development. Our preliminary results show that targeted deletion of focal adhesion kinase (FAK) in murine NCC results in cardiovascular and craniofacial alterations, resembling human congenital heart diseases The objective of this project is to elucidate the underlying mechanisms by which FAK mediates NCC function during development.
Compare gene expression in cranial neural crest cells of E11.5 wild type and FAK mutants.
FAK is a tyrosine kinase that transduces integrin and growth factor signaling pathways. Abnormal growth factor signaling leads to cardiovascular malformations caused by deficient cardiac NCC function. However, a direct role for FAK in NCC morphogenesis has not been demonstrated. We will test the hypothesis that FAK is a downstream target of essential growth factor signaling in cranial neural crest cells during development.
1) Generate E11.5 mouse embryos that are either control (FAK+/flox) or NCC specific FAK knockout (Wnt1Cre;FAKflox/flox). 2) Determine genotype by PCR. 3) Dissect head and torax from the different genotypes. 4) Obtain NCC from dissected tissue by magnetic cell sorting using p75 antibody. 5) Prepare total RNA from speciments. We will pool the NCC from 3 different embryos of the same genotype, and send total RNA to TGEN for probe preparation, hybridization and array result analysis.
Keywords: other
 
 
Contributor(s) Reichardt LF
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Submission date Apr 11, 2008
Last update date Feb 11, 2019
Contact name Winnie Liang
E-mail(s) wliang@tgen.org
Organization name Translational Genomics
Street address 445 N. Fifth Street
City Phoenix
State/province AZ
ZIP/Postal code 85012
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM281257 head/neck: E11.5FAKMut_le1
GSM281258 head/neck: E11.5FAKCtls_le1
GSM286147 head/neck: LM_Control_e1_le1
Relations
BioProject PRJNA106949

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11149_RAW.tar 29.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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