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| Status |
Public on May 30, 2008 |
| Title |
Hypothalamic gene expression data from Mecp2-null and MECP2-transgenic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We compared gene expression changes in the hypothalamus of mice lacking MeCP2 (Mecp2-null) and mice overexpressing MeCP2 (MECP2-transgenic). Mutations in the gene encoding the transcriptional repressor methyl-CpG binding protein 2 (MeCP2) cause the neurodevelopmental disorder Rett syndrome. Loss of function as well as increased dosage of MECP2 gene cause a host of neuropsychiatric disorders.
To explore the molecular mechanism(s) underlying these disorders, we examined gene expression patterns in the hypothalamus of mice that either lack or overexpress MeCP2. In both models, MeCP2 dysfunction induced changes in the expression levels of thousands of genes, but surprisingly the majority of genes (~85%) appeared to be activated by MeCP2. We selected six genes and confirmed that MeCP2 binds to their promoters. Furthermore, we showed that MeCP2 associates with the transcriptional activator CREB1 at the promoter of an activated target but not a repressed target. These studies suggest that MeCP2 regulates the expression of a wide range of genes in the hypothalamus and that it can function as both an activator and repressor of transcription.
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| Overall design |
Total hypothalamic RNA samples were collected from Mecp2-null male mice (n=4), MECP2-transgenic male mice (n=4), and their wild type male littermates at 6 weeks of age (n=4 for each group).
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| Contributor(s) |
Chahrour M, Jung S, Shaw CA, Zhou X, Wong ST, Qin J, Zoghbi HY |
| Citation(s) |
18511691 |
| |
| Submission date |
Apr 11, 2008 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Maria Chahrour |
| E-mail(s) |
chahrour@bcm.tmc.edu
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| Phone |
713-798-6695
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| Organization name |
Baylor College of Medicine
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| Department |
Molecular and Human Genetics
|
| Street address |
1 Baylor Plaza, T836
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77098 |
| Country |
USA |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (16)
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| GSM281260 |
Hypothalamus-wild type FVB-biological rep2 |
| GSM281261 |
Hypothalamus-wild type FVB-biological rep1 |
| GSM281262 |
Hypothalamus-MECP2-transgenic FVB-biological rep1 |
| GSM281263 |
Hypothalamus-MECP2-transgenic FVB-biological rep2 |
| GSM281264 |
Hypothalamus-MECP2-transgenic FVB-biological rep3 |
| GSM281265 |
Hypothalamus-wild type FVB-biological rep3 |
| GSM281266 |
Hypothalamus-wild type FVB-biological rep4 |
| GSM281267 |
Hypothalamus-MECP2-transgenic FVB-biological rep4 |
| GSM281268 |
Hypothalamus-Mecp2-null C57BL/6J-biological rep1 |
| GSM281269 |
Hypothalamus-wild type C57BL/6J-biological rep1 |
| GSM281270 |
Hypothalamus-Mecp2-null C57BL/6J-biological rep2 |
| GSM281271 |
Hypothalamus-wild type C57BL/6J-biological rep2 |
| GSM281272 |
Hypothalamus-wild type C57BL/6J-biological rep3 |
| GSM281273 |
Hypothalamus-wild type C57BL/6J-biological rep4 |
| GSM281274 |
Hypothalamus-Mecp2-null C57BL/6J-biological rep4 |
| GSM281275 |
Hypothalamus-Mecp2-null C57BL/6J-biological rep3 |
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| Relations |
| BioProject |
PRJNA106951 |
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