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Series GSE111702 Query DataSets for GSE111702
Status Public on Jun 05, 2018
Title The role of PKC-beta in B cell activation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary PKCb-null (Prkcb-/-) mice are severely immunodeficient. Here we show that mice whose B cells lack PKCb fail to develop germinal centers and plasma cells, which in concert undermine affinity maturation and antibody production in response to immunization. Moreover, Prkcb-/- B cells fail to differentiate into plasma cells in response to viral infection. At a cellular level, we show that activated Prkcb-/- B cells exhibit defective antigen polarization and mTORC1 signaling. While the loss of antigen polarization impairs antigen presentation and restricts the potential of Prkcb-/- B cells to develop into GC B cells, altered mTORC1 signaling affects gene expression, metabolic reprogramming and mitochondrial remodeling which together overwhelmingly oppose commitment to plasma cell differentiation. Mechanistically, we show that PKCb-mediated metabolic reprogramming is required for sustained heme biosynthesis that is essential for effector fate decision in B cells. Taken together, our study reveals mechanistic insights into the function of PKCb as a key regulator of B-cell polarity and metabolic reprogramming that instructs B-cell fate.
 
Overall design 10 samples (2x conditions; WT 3x biological replicate; KO 2x biological replicate)
 
Contributor(s) Tsui C, Martinez-Martin N, Gaya M, Maldonado P, Llorian M, Legrave M, Rossi M, MacRae J, Cameron A, Parker P, Leitges M, Bruckbauer A, batista F
Citation(s) 29884460
Submission date Mar 12, 2018
Last update date Jun 09, 2020
Contact name Carlson Tsui
E-mail(s) carlson.tsui@crick.ac.uk
Organization name The Francis Crick Institute
Street address 1 Midland Road
City London
ZIP/Postal code NW1 1AT
Country United Kingdom
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (10)
GSM3037771 WT1 non-stim d1
GSM3037772 WT2 non-stim d1
GSM3037773 WT3 non-stim d1
Relations
BioProject PRJNA437924
SRA SRP134939

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111702_Table_TPM_values_per_gene_per_sample.txt.gz 535.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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