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GEO help: Mouse over screen elements for information. |
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Status |
Public on Jun 05, 2018 |
Title |
The role of PKC-beta in B cell activation |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
PKCb-null (Prkcb-/-) mice are severely immunodeficient. Here we show that mice whose B cells lack PKCb fail to develop germinal centers and plasma cells, which in concert undermine affinity maturation and antibody production in response to immunization. Moreover, Prkcb-/- B cells fail to differentiate into plasma cells in response to viral infection. At a cellular level, we show that activated Prkcb-/- B cells exhibit defective antigen polarization and mTORC1 signaling. While the loss of antigen polarization impairs antigen presentation and restricts the potential of Prkcb-/- B cells to develop into GC B cells, altered mTORC1 signaling affects gene expression, metabolic reprogramming and mitochondrial remodeling which together overwhelmingly oppose commitment to plasma cell differentiation. Mechanistically, we show that PKCb-mediated metabolic reprogramming is required for sustained heme biosynthesis that is essential for effector fate decision in B cells. Taken together, our study reveals mechanistic insights into the function of PKCb as a key regulator of B-cell polarity and metabolic reprogramming that instructs B-cell fate.
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Overall design |
10 samples (2x conditions; WT 3x biological replicate; KO 2x biological replicate)
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Contributor(s) |
Tsui C, Martinez-Martin N, Gaya M, Maldonado P, Llorian M, Legrave M, Rossi M, MacRae J, Cameron A, Parker P, Leitges M, Bruckbauer A, batista F |
Citation(s) |
29884460 |
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Submission date |
Mar 12, 2018 |
Last update date |
Jun 09, 2020 |
Contact name |
Carlson Tsui |
E-mail(s) |
carlson.tsui@crick.ac.uk
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Organization name |
The Francis Crick Institute
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Street address |
1 Midland Road
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City |
London |
ZIP/Postal code |
NW1 1AT |
Country |
United Kingdom |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (10)
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Relations |
BioProject |
PRJNA437924 |
SRA |
SRP134939 |
Supplementary file |
Size |
Download |
File type/resource |
GSE111702_Table_TPM_values_per_gene_per_sample.txt.gz |
535.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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