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| Status |
Public on Mar 28, 2018 |
| Title |
RNA-seq of bone marrow derived macrophages stimulated with monophosphoryl lipid A (MPLA) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We found that MPLA reprograms macrophages in a way that supports a persistent monocyte/macrophage chemokine secretion profile reflected in macrophage mRNA. Additionally, this RNA-seq data revealed that certain genes (e.g. phagocytosis-related) persist much longer after MPLA than others (e.g. pro-inflammatory cytokines).
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| Overall design |
Bone marrow derived macrophages were harvested for RNA after 4hrs of monophosphoryl lipid A (MPLA) priming, 24hrs of MPLA priming, and 3 days following the end of priming
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| Contributor(s) |
Fensterheim BA, Bohannon JK |
| Citation(s) |
29686054 |
| |
| Submission date |
Mar 12, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Benjamin Ari Fensterheim |
| E-mail(s) |
benjamin.a.fensterheim@vanderbilt.edu
|
| Phone |
3012375083
|
| Organization name |
Vanderbilt University
|
| Department |
Pathology, Microbiology, and Immunology
|
| Lab |
Sherwood Lab
|
| Street address |
1161 21st Avenue South
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA437979 |
| SRA |
SRP134976 |
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