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| Status |
Public on Sep 14, 2018 |
| Title |
THE DNA BINDING LANDSCAPE OF THE MAIZE AUXIN RESPONSE FACTOR FAMILY [DAP-seq] |
| Organism |
Zea mays |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
DAP-seq was used to generate genome-wide DNA:TF interaction maps for fourteen maize ARFs from the evolutionarily conserved class A ‘activator’ and class B ‘repressor’ clades. Among the distinct binding sites that were identified, we observed a high degree of overlap for ARFs of the same class, but found substantial differences in motif sequence, spacing, site preference, and association with auxin induced genes among clade A and clade B ARFs.
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| Overall design |
Genome-wide DNA binding events were determined for fourteen maize ARFs using DAP-seq. Purified genomic DNA from B73 was incubated with purified GST-ARF fusion proteins expressed in Ecoli.
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| Contributor(s) |
Galli M, Gallavotti A |
| Citation(s) |
30375394 |
| Submission date |
Mar 13, 2018 |
| Last update date |
Dec 14, 2018 |
| Contact name |
Andrea Gallavotti |
| E-mail(s) |
agallavotti@waksman.rutgers.edu
|
| Organization name |
Rutgers University
|
| Department |
Waksman Institute
|
| Lab |
Gallavotti Lab
|
| Street address |
190 Frelinghuysen Rd
|
| City |
Piscataway |
| State/province |
New Jersey |
| ZIP/Postal code |
08854-8020 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20156 |
Illumina NextSeq 500 (Zea mays) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE111857 |
THE DNA BINDING LANDSCAPE OF THE MAIZE AUXIN RESPONSE FACTOR FAMILY |
|
| Relations |
| BioProject |
PRJNA438151 |
| SRA |
SRP135513 |
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