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| Status |
Public on Mar 01, 2020 |
| Title |
Kidney-resident CD8 T cells downregulate IL-18R during maturation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using the expression of IL-18 receptor (CD218) as a surface marker, CD69+ kidney-resident CD8 T cells can be separated into two subsets. Transcriptional analysis suggests that IL-18R low subset represents the mature tissue-resident T cells
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| Overall design |
10000 congenically marked naïve P14 T cells were adoptively transferred into each sex-matched unmanipulated C57BL/6 mouse followed by LCMV Armstrong infection via an intraperitoneal route. 12 days after infection, lymphocytes were purified from the kidneys via enzymatic digestion and gradient centrifugation. Indicated P14 T cell subsets were FACS sorted from purified total kidney lymphocytes. Total RNA was extracted from freshly sorted cells. Kidneys from 10-15 recipient mice were pooled for each sorting.
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| Contributor(s) |
Nu Z, Lai Z, Ma C, Liu Y, Demel E, Liu X, Mishra S, Lu Q, Liao W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 14, 2018 |
| Last update date |
Mar 02, 2020 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
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| Phone |
2105629163
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| Organization name |
UT Health Science Center at San Antonio
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| Department |
Population Health Sciences
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| Street address |
8403 Floyd Curl Drive, MSC 7784
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| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA438210 |
| SRA |
SRP135646 |
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