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| Status |
Public on Mar 30, 2019 |
| Title |
Sialic acid relieves RetS-dependent inhibition of GacS to induce a chronic infection state of Pseudomonas aeruginosa |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The purpose of this experiment was to see if the addition of sialic acid activated the GacS-GacA regulon, which includes >300 genes.
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| Overall design |
Cultures were grown to OD~0.6, and then split in half. 2 mM sialic acid was added to one culture but not to the other. After 20 minutes of incubation with sialic acid, RNA was extracted from the + or - sialic acid samples for RNA-seq.
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| Contributor(s) |
Wang BX, Cady KC, Ribbeck K, Laub MT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 15, 2018 |
| Last update date |
Mar 30, 2019 |
| Contact name |
Benjamin Wang |
| Organization name |
MIT
|
| Street address |
31 Ames Street
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02141 |
| Country |
USA |
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| Platforms (1) |
| GPL18644 |
Illumina HiSeq 2000 (Pseudomonas aeruginosa) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA438513 |
| SRA |
SRP135806 |
