|
| Status |
Public on Apr 25, 2019 |
| Title |
Genome-wide binding of the meiosis I-specific Spo13 in the presence and absence of cohesin |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP-seq profile of Spo13 binding sites arrested in prophase I by deletion of the meiotic transcription factor NDT80. Previous ChIP-ChIP data had indicated that Spo13 binding correlates with cohesin binding sites. Therefore, in addition to wild type cells, we also investigated cells deficient of the meiosis-specific cohesin subunit Rec8 to identify cohesin-dependent binding sites of Spo13.
|
| |
|
| Overall design |
Diploid S. cerevisiae SK1 cells were constructed containing Spo13-3Flag in an ndt80Δ background. Cells were allowed to sporulate in sporulation media for 5 hours after which formaldehyde was added to the cultures and cells were fixed for 30min. Samples were then processed according to the standard lab ChIP-Seq protocol.
|
| |
|
| Contributor(s) |
Galander S, Marston AL |
| Citation(s) |
31031198 |
| |
| Submission date |
Mar 21, 2018 |
| Last update date |
Jun 21, 2019 |
| Contact name |
Daniel Robertson |
| E-mail(s) |
daniel.robertson@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Discovery Research Platform for Hidden Cell Biology
|
| Lab |
Bioinformatics Core
|
| Street address |
2.28 Michael Swann Building, Kings Buildings, Mayfield Road
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL22715 |
Illumina MiniSeq (Saccharomyces cerevisiae) |
|
| Samples (4)
|
| GSM3059109 |
AM24162 Spo13-3Flag ChIP-seq rec8Δ Input |
|
| This SubSeries is part of SuperSeries: |
| GSE112217 |
Reductional meiosis I chromosome segregation is established by coordination of key meiotic kinases |
|
| Relations |
| BioProject |
PRJNA442754 |
| SRA |
SRP136197 |
Less...
More...