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Status |
Public on Jun 28, 2018 |
Title |
Nucleotide resolution genome-wide mapping of 8-oxoG damage |
Organism |
Saccharomyces cerevisiae BY4741 |
Experiment type |
Other
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Summary |
Single nucleotide resolution sequencing of DNA damage is required to decipher the complex causal link between the identity and location of DNA adduct and mutation patterns in the genome. We coupled the specificity of the repair enzyme with the efficiency of a one-pot click-DNA ligation reaction to insert a readable oligonucleotide code sequence. The biocompatible code enabled high-throughput, base resolution sequencing of the 8-oxoguanine site and thus have named the method click-code-seq. By applying click-code-seq to a eukaryotic (yeast) genome, we uncovered thousands of 8-oxoguanine sites with features and patterns suggesting a potential relationship to chromatin formation and transcription.
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Overall design |
We sequenced the yeast (BY4741) genome during exponential growth in rich media to generate a nucleotide-resolution background map of 8-oxoG damage.
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Contributor(s) |
Wu J, Sturla SJ |
Citation(s) |
29944356 |
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Submission date |
Mar 22, 2018 |
Last update date |
Jun 30, 2018 |
Contact name |
Shana J. Sturla |
E-mail(s) |
sturlas@ethz.ch
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Organization name |
ETH Zurich
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Department |
Department of Health Sciences and Technology
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Street address |
Schmelzbergstrasse 9
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City |
Zurich |
ZIP/Postal code |
8092 |
Country |
Switzerland |
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Platforms (1) |
GPL21372 |
Illumina MiSeq (Saccharomyces cerevisiae BY4741) |
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Samples (3) |
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Relations |
BioProject |
PRJNA445275 |
SRA |
SRP136249 |