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Series GSE112234 Query DataSets for GSE112234
Status Public on Apr 28, 2020
Title In vitro capture and characterization of embryonic rosette-stage pluripotency between naive and primed states
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Upon implantation, the naive pluripotent epiblast of the mouse blastocyst generates a rosette, undergoes lumenogenesis and forms the primed pluripotent egg cylinder, able to generate the embryonic tissues. How pluripotency progression and morphogenesis are linked, and whether intermediate pluripotent states exist remain controversial. We identify here a rosette pluripotent state, defined by co-expression of naive factors with transcription factor OTX2. Downregulation of blastocyst WNT signals drives transition into rosette pluripotency by inducing OTX2. The rosette then activates MEK signals that induce lumenogenesis and drive progression to primed pluripotency. Consequently, combined WNT and MEK inhibition supports rosette-like stem cells (RSCs), a self-renewing naive-primed intermediate. RSCs erase constitutive heterochromatin marks and display a primed chromatin landscape, with bivalently marked primed pluripotency genes. Nonetheless, WNT induces reversion to naive pluripotency. The rosette is therefore a reversible pluripotent intermediate where control over both pluripotency progression and morphogenesis pivots from WNT to MEK signals.
 
Overall design Examination of H3K27me3 and H3K4me3 in various mouse pluripotent states. Comparison with previously published 2i ESCs and EpiSCs.
 
Contributor(s) Marks H, Dirks RA, van Mierlo G, Neagu A, van Genderen E, Escudero I, Verwegen L, Kurek D, Lehmann J, Stel J, Maas A, Eleveld C, Ge Y, den Dekker A, Brouwer RW, van Ijcken WF, Modic M, Drukker M, Jansen JH, Rivron NC, Baart E, ten Berge D
Citation(s) 32367046
Submission date Mar 22, 2018
Last update date Jul 28, 2020
Contact name Hendrik Marks
E-mail(s) h.marks@ncmls.ru.nl
Organization name Radboud University Nijmegen, RIMLS
Department Molecular Biology
Street address Geert Grooteplein 26/28
City Nijmegen
ZIP/Postal code 6525GA
Country Netherlands
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM3061432 RSC-ChIP-CGR8-H3K27me3
GSM3061433 RSC-ChIP-CGR8-H3K4me3
GSM3061434 RSC-ChIP-R1-H3K27me3
Relations
BioProject PRJNA445312
SRA SRP136281

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Supplementary file Size Download File type/resource
GSE112234_RAW.tar 540.8 Mb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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