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| Status |
Public on Jun 21, 2022 |
| Title |
Reinforcement of DNA methylation in CHH context through RNA-directed DNA methylation ensures sexual reproduction in rice [BS-Seq] |
| Organism |
Oryza sativa |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
DNA methylation is an important epigenetic mark that regulates the expression of genes and transposons. RNA-directed DNA methylation (RdDM) is the main molecular pathway responsible for de novo DNA methylation in plants. Although the mechanism of RdDM has been well understood in Arabidopsis, mutations in RdDM genes cause no visible developmental defects in Arabidopsis. Here, we isolated and cloned Five Elements Mountain 1 (FEM1), which encodes RNA-dependent RNA polymerase 2 in rice. Mutation in OsRDR2 abolished the accumulation of 24-nt small interfering RNAs, and consequently substantially decreased genome-wide CHH methylation levels. Moreover, male and female reproductive development was disturbed, which led to the sterility of osrdr2 mutants. We discovered that OsRDR2-dependent DNA methylation likely regulates the expression of multiple key genes involved in stamen development and meiosis. In wild-type (WT) plants but not in osrdr2 mutants, genome-wide CHH DNA methylation levels were greater in panicles, stamens, and pistils than in seedlings. The global increase of methylation in reproductive organs of the WT was mainly explained by the enhancement of RdDM activity including OsRDR2 activity. Our results, which revealed a global increase in DNA methylation through enhancement of RdDM activity in reproductive organs, demonstrate the crucial role of OsRDR2 in the sexual reproduction of rice.
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| Overall design |
whole genome wide DNA methylation data by bisulphite sequencing
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| Contributor(s) |
Wang L, Yang D |
| Citation(s) |
34791444 |
| Submission date |
Mar 23, 2018 |
| Last update date |
Jun 22, 2022 |
| Contact name |
Lili Wang |
| E-mail(s) |
2014201023@njau.edu.cn
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| Organization name |
Nanjing Agricultural University
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| Street address |
Weigang No.1
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| City |
Nanjing |
| ZIP/Postal code |
210095 |
| Country |
China |
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| Platforms (1) |
| GPL23013 |
Illumina HiSeq 4000 (Oryza sativa) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE112259 |
Reinforcement of DNA methylation in CHH context through RNA-directed DNA methylation ensures sexual reproduction in rice |
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| Relations |
| BioProject |
PRJNA445402 |
| SRA |
SRP136330 |
