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Series GSE112321 Query DataSets for GSE112321
Status Public on Nov 28, 2018
Title Extrinsic C5a Activation Regulates Squamous Carcinogenesis and Limits Clonal T Cell Responses to Chemotherapy (Blood)
Organism Mus musculus
Experiment type Other
Summary In the K14-HPV16 transgenic mouse model of squamous carcinogenesis, activation of C5a receptor (C5aR1) in early neoplastic tissues fosters protumorigenic properties of C5aR1+ mast cells and macrophages, and in turn, suppression of CD8+ T cell cytotoxicity. Therapeutic inhibition of C5a receptor (C5aR1) with a peptide antagonist improved efficacy to paclitaxel chemotherapy associated with CXCR3-dependent CD8+ T cell activation. To investigate the effects of combination therapy on the T cell repertoire, we performed deep sequencing of the complementarity-determining region (CDR) 3 of the T cell receptor (TCR)b chain in matched tumor lysates and peripheral blood mononuclear cells (PBMCs). Intratumoral TCR╬▓ clonotypes were hyperexpanded and increasingly detected in matched peripherally-expanded T cell populations, thereby implicating antigen-dependent peripheral priming in response to systemic C5aR1 inhibition.
 
Overall design Deep sequencing of the complementarity-determining region (CDR) 3 of the T cell receptor (TCR)b chain in matched tumor lysates and peripheral blood mononuclear cells (PBMCs)
 
Contributor(s) Medler T, Murugan D, Horton W, Kumar S, Cotechini T, Forsyth A, Leyshock P, Leitenberger J, Kulesz-Martin M, Margolin A, Werb Z, Coussens L
Citation(s) 30300579
Submission date Mar 26, 2018
Last update date Jan 30, 2020
Contact name Lisa Coussens
E-mail(s) coussenl@ohsu.edu
Organization name OHSU
Street address SW Sam Jackson Park Rd
City Portland
State/province Oregon
ZIP/Postal code 97239
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (44)
GSM3067656 untreated control 1 (blood)
GSM3067657 untreated control 2 (blood)
GSM3067658 untreated control 3 (blood)
This SubSeries is part of SuperSeries:
GSE112323 Extrinsic C5a Activation Regulates Squamous Carcinogenesis and Limits Clonal T Cell Responses to Chemotherapy
Relations
BioProject PRJNA445687
SRA SRP136451

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112321_RAW.tar 144.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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